Influence of Meloidogyne javanica and Rotylenchulus reniformis alone on nodulation of some leguminous crops
S.
Montasser
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Cairo.
author
N.
Mahmoud
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch
author
A.
El-Mesalamy
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch
author
M.
Abdel-Mageed
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch
author
text
article
2016
eng
The effect of root-knot nematode, Meloidogyne javanica and the reniformnematode, Rotylenchulus reniformis alone on nodulation of four Rhizobium specieson roots of 27 cultivars related to six leguminous species were evaluated. Theformation of nodules on their roots varied greatly in their numbers according to hostspecies, host cultivars, rhizobal species and nematode species. The greaternodulation reduction in infected roots with nematode species, M. javanica and R.reniformis was observed on roots of common bean cvs. Exira, Nebraska, Polistaand Savana inoculated with rhizobial species Rhizobium phaseoli and lupine cv.Giza 2 inoculated with rhizobial species R. lupine. In general, inoculation of M.javanica or R. reniformis with rhizobial species greatly reduced root nodulation ascompared to leguminous species inoculated with Rhizobium species alone. Also, itis important to note that the reduction in the number of bacterial nodules was morepronounced with R. reniformis infection than with M. javanica infection. It isnoteworthy that the number of nodules on roots of broad bean cv. Wade 1inoculated with R. phaseoli increased by both nematode species infections.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
1
11
https://ejaj.journals.ekb.eg/article_57476_e4063175a4abbeffff94b0ead6452485.pdf
dx.doi.org/10.21608/ejaj.2016.57476
Efficacy of some agro-wastes of date palm as soil amendment for management of Meloidogyne javanica and Rotylenchulus reniformis on common bean
S.
Montasser
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Cairo.
author
N.
Mahmoud
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.
author
A.
El-Mesalamy
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.
author
M.
Abdel-Mageed
Agric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.
author
text
article
2016
eng
The efficacy of date palm (Phoenix dactylifera L.) agro-wastes viz. palmfibers, green drop fruits, palm fronds and rules of leaves applied as powder orsoakage to soil at various rates of application were evaluated for their nematisidalactivates against root-knot nematode, Meloidogynejavanicaand reniform nematode,Rotylenchulusreniformis infected common bean (Phaseolus vulgaris L.) cv. Polistaunder greenhouse conditions. All the tested soil amendments of date palm wastessignificantly reduced the number of nematode in soil, developmental stages andegg-masses per root for both nematode species as well as the number of root gallsformed by root-knot nematode on common bean roots when compared withuntreated check. Also, the percentage values of reductions of nematode finalpopulation and egg production as well as the rate of nematode reproductiongradually decreased with increasing the amount of agro-wastes of date palm to thesoil. The agro-wastes of green drop fruits and rules of leaves applied as powder orsoakage at the rates of 15 and 20 g/kg soil proved to be the best effect in controllingfor both nematode species than those of the other tested treatments. It is worthy tonot that the effect of the powder of agro-wastes of date palm on M. javanicareproduction was more than the soaking agro-wastes while the soaking agro-wasteson nematode reproduction of R. reniformis was more effective than powder agrowastes.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
12
28
https://ejaj.journals.ekb.eg/article_57477_2685c4f5700c7d55f5eae7b1e623058b.pdf
dx.doi.org/10.21608/ejaj.2016.57477
Potential Use of Botanicals Rich in Alkaloids for Controlling Meloidogyne incognita and Rotylenchulus reniformis Infecting Cucurbits
Fatma
Mostafa
Nematology Research Unit, Agric. Zool. Dept., Fac. Agric., Mansoura Univ., Mansoura. Egypt
author
A.
Refaei
Nematology Research Unit, Agric. Zool. Dept., Fac. Agric., Mansoura Univ., Mansoura. Egypt
author
A.
Khalil
Plant Pathol. Res. Inst., Agric. Res. Center, Giza, Egypt.
author
Marwa
El-Deriny
Plant Pathol. Res. Inst., Agric. Res. Center, Giza, Egypt.
author
text
article
2016
eng
Two greenhouse experiments were conducted to evaluate the nematicidalactivity of certain alkaloid plants namely, periwinkle (Catharanthus roseus), datura(Datura stramonium), lupin (Lupinus albus), pomegranate (Punica granatum) andfenugreek (Trigonella foenum-graecum) against root-knot nematode Meloidogyneincognita infecting cucumber (Cucumis sativus) and reniform nematode(Rotylenchulus reniformis) infecting cantaloupe (Cucumis melo var.cantaloupensis).Dried powdered parts of such plants were screened at three rates (1, 3 and 5g /plant). In most treatments data indicated a positive correlation between tested ratesand plant biomass. Dried leaf powder of C. roseus (62.5%) as well as dried seedpowder of L. albus ( 52.8%), and T. foenum-graecum ( 43.2 %) induced remarkableimprovement in total plant fresh weight of cucumber at the rate of 5g/plant.However, dried leaf powder of D. stramonium (41.4%) recorded a significantincrease in total plant fresh weight at the rate of 3g/plant. By all treatments ofalkaloid plants root-knot nematode population whether in soil and roots, root gallingand number of egg masses were significantly suppressed. Dried leaf powder of C.roseus at three tested rates (71.8, 71.6 and 72.6%) performed the best andsuppressed total nematode population of M. incognita followed by T. foenumgraceumat 5g/plant (70.1%). On the other hand, a slight to moderate improvementin plant growth parameters of cantaloupe infected with R. reniformis was indicatedin pots receiving alkaloids plants. The highest percentage of increase in total plantfresh weight was noticed with dried leaf powder of C. roseus at three tested rates(28.5, 31.4 and 32.6%) respectively. The best protection of cantaloupe against R.reniformis was recorded with dried seed powder of T. foenum-graecum (57.9, 65.2and 75.0%) and P. granatum (55.4, 55.9 and 61.7%) at three tested rates,respectively which ultimately suppressed R. reniformis population.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
29
43
https://ejaj.journals.ekb.eg/article_57478_7dafd0c77d18abd8179f2822fc7ee9fd.pdf
dx.doi.org/10.21608/ejaj.2016.57478
Electrophoretic Patterns of Some Populations of Meloidogyne Arenaria and M. javanica
Mahfouz
Abd-Elgawad
Phytopathology Department, National Research Centre;
author
Mohamed
Eissa
Phytopathology Department, National Research Centre;
author
Abd-Elmoneim
El-Gindi
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, Egypt
author
Ahmed
Osman
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, Egypt
author
Grover
Smart
Department of Entomology and Nematology, IFAS, University of Florida, USA
author
Ahmed
El-bahrawy
Institute for Sustainable Plant Protection, National Council of Research, Bari, Italy.
author
text
article
2016
eng
Root-knot nematodes (Meloidogyne spp.) are important polyphagous plantparasites worldwide. Intraspecific variation and the occurrence of physiologicalraces have necessitated the development of taxonomic techniques enablingdifferentiation and classification of races related to major Meloidogyne spp.Accurate identification of their races and populations is essential for determiningappropriate management approaches and benefits from further characterization andunderstanding of the variability within a single nematode species. Electrophoreticfocusing of malic dehydrogenase, α-glycerophosphate dehydrogenase andesterase for seven populations of Meloidogyne spp. by polyacrylamide gels wastested. These populations included 1, 2 and 4 populations of M. javanica and M.arenaria race I and II, respectively. Malic Dehydrogenase profiles obtained bypolyacrylamide-gel electrophoresis indicated that differences between Meloidogynearenaria and M. javanica were more pronounced than were differences betweenraces or populations of M. arenaria. The enzyme patterns of the two species variedeven though the nematodes were propagated on the same host plant (Rutgerstomato).
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
44
52
https://ejaj.journals.ekb.eg/article_57479_966c1362dc8a974fa25d4a5d45cefae6.pdf
dx.doi.org/10.21608/ejaj.2016.57479
Long-term effect of Tylenchulus semipenetrans on citrus tree quality in reclaimed land of Egypt
M.
Abd-Elgawad
Phytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, Egypt
author
F.
Koura
Phytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, Egypt
author
S.
Montasser
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, Al-Azhar University, Egypt,
author
M.
Hammam
Phytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, Egypt
author
Ahmed
El-bahrawy
Institute for Sustainable Plant Protection, National Council of Research, Bari, Italy.
author
text
article
2016
eng
Citrus fruit yield is considered a short-term response to the citrus nematode,Tylenchulus semipenetrans damage and therefore this study addressed parametersmeasuring overall appearance of the sampled trees. Soil and roots from 20 trees ineach of three mature: 'banzaheer' lemon, 'Baladi' mandarin, and ‘Navel’ orange andone immature: ‘Navel’ orange orchards, all grafted on sour orange rootstock, inEgypt were sampled for the citrus nematode, T. semipenetrans, in February 2011 toinvestigate their correlations with tree parameters (tree height and vigor, canopydiameter, and trunk circumference) that reflect long-term nematode damage of thesampled trees. The correlation between pairs of these four parameters were alwayspositive and generally with high significant levels. Each of the four parameters wasnot significantly correlated with fruit yield of the mature trees but each of tree heightand vigor, and canopy diameter was correlated with fruit yield of the immature Navelorange. The relationships between pairs of these biotic parameters and T.semipenetrans population density in fibrous roots and soil were not consistent.Eleven edaphic factors in mandarin and mature orange orchards were measuredand compared. Both soils were dominated by rough grainy particles of sand. Levelsof potassium, calcium, phosphorus, ammonium and nitrate as well as soil solutionpH, organic matter content and electric conductivity in the soil samples weresignificantly (P ≤ 0.01) different between the mandarin and orange orchards. Toachieve better economic revenue of citriculture, local tackling of T. semipenetransproblem in newly reclaimed areas of Egypt was discussed.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
53
66
https://ejaj.journals.ekb.eg/article_57482_b84bdf3d826ae2b733837b90f833c766.pdf
dx.doi.org/10.21608/ejaj.2016.57482
Nematicidal properties of some algal aqueous extracts against root-knot nematode, Meloidogyne incognita in vitro
Ahmed
Nour El-Deen
Nematology Research Unit, Agricultural Zoology Dept., Faculty of Agriculture, Mansoura University, Egypt
author
Ahmed
Issa
Department of Botany, Faculty of Science, Assiut University, Assiut , Egypt
author
text
article
2016
eng
The effectiveness of aqueous extracts derived from nine algal species atdifferent concentrations on egg hatching and mortality of Meloidogyne incognita(Kofoid and White) Chitwood juveniles after various exposure times weredetermined in vitro. Results indicated that Enteromorpha flexuosa at theconcentration of 80% was the best treatment for suppressing the egg hatching withvalue of 2 % after 5 days of exposure, followed by Dilsea carnosa extract (3%) andCodium fragile (4%) at the same concentration and exposure time. Likewise,application of C. fragile, D. carnosa , E. flexuosa and Cystoseira myrica extracts atthe concentrations of 80 and 60% were highly toxic to the nematodes, killing morethan 90 % of nematode larva after 72 hours of exposure while the others gave quitelow mortalities. The characteristic appearances in shape of the nematodes killed byC. fragile, D. carnosa , C. myrica, E. flexuosa and Sargassum muticum wassigmoid (Σ-shape) with some curved shape; whereas, the nematodes killed by otheralgal species mostly followed straight or bent shapes. The present study proved thatfour species of algae C. fragile, D. carnosa, C. myrica and E. flexuosa could beused for the bio-control of root-knot nematodes.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
67
78
https://ejaj.journals.ekb.eg/article_57484_028d59078e007eb70d59acaeb82d652a.pdf
dx.doi.org/10.21608/ejaj.2016.57484
Efficacy of some Bioagents, Algal Species and Plant Extracts in Controlling Meloidogyne Incognita on Yield of Grape Vines in Egypt
Samaa
Shawky
Nematode Research Department, Plant Pathology Research Institute, Agricultural Research Center,
Giza, Egypt
author
Ahlam
Al-Ghonaimy
Plant Protection Department, Nematology Unit, Desert Research Center, Cairo, Egypt
author
Eman
Hammad
Nematode Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
author
text
article
2016
eng
These experiments were conducted to control the root-knot nematodes,Meloidogyne incognita under both greenhouse and field conditions in superiorseedless vineyards and its reflection on yield. The field experiment was carried outin a private vineyard (El Wady El Faregh) in Alexandria-Cairo road (kilo 78). Seventreatments were used as follows: two bioagents (Bacillus subtilis and Trichodermaharzianum), two blue green strains algal species (Cyanobacteria) (Spirulinaplatensis and Oscillatoria sp.) and two plant aqueous extracts (Ambrosia maritimeand Eucalyptus globules) at three concentrations, and in comparison with oxamyl(24% EC) were used to control Meloidogyne incognita on superior seedlessvineyards on grapes under both greenhouse and field conditions. Bioagents, algalspecies and plant extracts were added to the soil under vines one time weekly forthree weeks after bud burst under both greenhouse and field conditions.The most effective treatments in controlling root-knot nematodes;Meloidogyne incognita were both Spirulina platensis and Oscillatoria sp whereasthe least effective was suspension of Eucalyptus globules under both greenhouseand field conditions. Both Spirulina platensis and Oscillatoria sp acheived thehighest reduction effect on number of 2nd stage juveniles in soil, numbers of galls,developmental stages, females, egg masses, egg numbers/egg mass in roots andfinal population Meloidogyne incognita on grape plants, whereas the suspension ofEucalyptus globules was the least effective treatment under both greenhouse andfield conditions. In addition all treatments increased fresh weight of the whole plantover control under greenhouse conditions.Results showed that all the treatments had a significant effect in reducing thetotal population and build up of root – knot nematodes, Meloidogyne incognita inboth soil and roots especially after three months from the time of application. Inaddition, all treatments had increased the yield of superior seedless grapevinesunder field conditions.
Egyptian Journal of Agronematology
Egyptian Society for Agronematology
1110-6158
15
v.
1
no.
2016
79
94
https://ejaj.journals.ekb.eg/article_57485_7223526125e8c0ec638c12da390684fd.pdf
dx.doi.org/10.21608/ejaj.2016.57485