Egyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Influence of Meloidogyne javanica and Rotylenchulus reniformis alone on nodulation of some leguminous crops1115747610.21608/ejaj.2016.57476ENS.MontasserAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Cairo.N.MahmoudAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut BranchA.El-MesalamyAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut BranchM.Abdel-MageedAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut BranchJournal Article20191107The effect of root-knot nematode, Meloidogyne javanica and the reniform<br />nematode, Rotylenchulus reniformis alone on nodulation of four Rhizobium species<br />on roots of 27 cultivars related to six leguminous species were evaluated. The<br />formation of nodules on their roots varied greatly in their numbers according to host<br />species, host cultivars, rhizobal species and nematode species. The greater<br />nodulation reduction in infected roots with nematode species, M. javanica and R.<br />reniformis was observed on roots of common bean cvs. Exira, Nebraska, Polista<br />and Savana inoculated with rhizobial species Rhizobium phaseoli and lupine cv.<br />Giza 2 inoculated with rhizobial species R. lupine. In general, inoculation of M.<br />javanica or R. reniformis with rhizobial species greatly reduced root nodulation as<br />compared to leguminous species inoculated with Rhizobium species alone. Also, it<br />is important to note that the reduction in the number of bacterial nodules was more<br />pronounced with R. reniformis infection than with M. javanica infection. It is<br />noteworthy that the number of nodules on roots of broad bean cv. Wade 1<br />inoculated with R. phaseoli increased by both nematode species infections.<br /><br />https://ejaj.journals.ekb.eg/article_57476_e4063175a4abbeffff94b0ead6452485.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Efficacy of some agro-wastes of date palm as soil amendment for management of Meloidogyne javanica and Rotylenchulus reniformis on common bean12285747710.21608/ejaj.2016.57477ENS.MontasserAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Cairo.N.MahmoudAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.A.El-MesalamyAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.M.Abdel-MageedAgric. Zool. and Nematol. Dept., Fac. Agric. Al-Azhar University. Assiut Branch.Journal Article20191107The efficacy of date palm (Phoenix dactylifera L.) agro-wastes viz. palm<br />fibers, green drop fruits, palm fronds and rules of leaves applied as powder or<br />soakage to soil at various rates of application were evaluated for their nematisidal<br />activates against root-knot nematode, Meloidogynejavanicaand reniform nematode,<br />Rotylenchulusreniformis infected common bean (Phaseolus vulgaris L.) cv. Polista<br />under greenhouse conditions. All the tested soil amendments of date palm wastes<br />significantly reduced the number of nematode in soil, developmental stages and<br />egg-masses per root for both nematode species as well as the number of root galls<br />formed by root-knot nematode on common bean roots when compared with<br />untreated check. Also, the percentage values of reductions of nematode final<br />population and egg production as well as the rate of nematode reproduction<br />gradually decreased with increasing the amount of agro-wastes of date palm to the<br />soil. The agro-wastes of green drop fruits and rules of leaves applied as powder or<br />soakage at the rates of 15 and 20 g/kg soil proved to be the best effect in controlling<br />for both nematode species than those of the other tested treatments. It is worthy to<br />not that the effect of the powder of agro-wastes of date palm on M. javanica<br />reproduction was more than the soaking agro-wastes while the soaking agro-wastes<br />on nematode reproduction of R. reniformis was more effective than powder agrowastes.<br /><br />https://ejaj.journals.ekb.eg/article_57477_2685c4f5700c7d55f5eae7b1e623058b.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Potential Use of Botanicals Rich in Alkaloids for Controlling Meloidogyne incognita and Rotylenchulus reniformis Infecting Cucurbits29435747810.21608/ejaj.2016.57478ENFatmaMostafaNematology Research Unit, Agric. Zool. Dept., Fac. Agric., Mansoura Univ., Mansoura. EgyptA.RefaeiNematology Research Unit, Agric. Zool. Dept., Fac. Agric., Mansoura Univ., Mansoura. EgyptA.KhalilPlant Pathol. Res. Inst., Agric. Res. Center, Giza, Egypt.MarwaEl-DerinyPlant Pathol. Res. Inst., Agric. Res. Center, Giza, Egypt.Journal Article20191107Two greenhouse experiments were conducted to evaluate the nematicidal<br />activity of certain alkaloid plants namely, periwinkle (Catharanthus roseus), datura<br />(Datura stramonium), lupin (Lupinus albus), pomegranate (Punica granatum) and<br />fenugreek (Trigonella foenum-graecum) against root-knot nematode Meloidogyne<br />incognita infecting cucumber (Cucumis sativus) and reniform nematode<br />(Rotylenchulus reniformis) infecting cantaloupe (Cucumis melo var.cantaloupensis).<br />Dried powdered parts of such plants were screened at three rates (1, 3 and 5g /<br />plant). In most treatments data indicated a positive correlation between tested rates<br />and plant biomass. Dried leaf powder of C. roseus (62.5%) as well as dried seed<br />powder of L. albus ( 52.8%), and T. foenum-graecum ( 43.2 %) induced remarkable<br />improvement in total plant fresh weight of cucumber at the rate of 5g/plant.<br />However, dried leaf powder of D. stramonium (41.4%) recorded a significant<br />increase in total plant fresh weight at the rate of 3g/plant. By all treatments of<br />alkaloid plants root-knot nematode population whether in soil and roots, root galling<br />and number of egg masses were significantly suppressed. Dried leaf powder of C.<br />roseus at three tested rates (71.8, 71.6 and 72.6%) performed the best and<br />suppressed total nematode population of M. incognita followed by T. foenumgraceum<br />at 5g/plant (70.1%). On the other hand, a slight to moderate improvement<br />in plant growth parameters of cantaloupe infected with R. reniformis was indicated<br />in pots receiving alkaloids plants. The highest percentage of increase in total plant<br />fresh weight was noticed with dried leaf powder of C. roseus at three tested rates<br />(28.5, 31.4 and 32.6%) respectively. The best protection of cantaloupe against R.<br />reniformis was recorded with dried seed powder of T. foenum-graecum (57.9, 65.2<br />and 75.0%) and P. granatum (55.4, 55.9 and 61.7%) at three tested rates,<br />respectively which ultimately suppressed R. reniformis population.<br /><br />https://ejaj.journals.ekb.eg/article_57478_7dafd0c77d18abd8179f2822fc7ee9fd.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Electrophoretic Patterns of Some Populations of Meloidogyne Arenaria and M. javanica44525747910.21608/ejaj.2016.57479ENMahfouzAbd-ElgawadPhytopathology Department, National Research Centre;MohamedEissaPhytopathology Department, National Research Centre;Abd-ElmoneimEl-GindiDepartment of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, EgyptAhmedOsmanDepartment of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, EgyptGroverSmartDepartment of Entomology and Nematology, IFAS, University of Florida, USAAhmedEl-bahrawyInstitute for Sustainable Plant Protection, National Council of Research, Bari, Italy.Journal Article20191107Root-knot nematodes (Meloidogyne spp.) are important polyphagous plant<br />parasites worldwide. Intraspecific variation and the occurrence of physiological<br />races have necessitated the development of taxonomic techniques enabling<br />differentiation and classification of races related to major Meloidogyne spp.<br />Accurate identification of their races and populations is essential for determining<br />appropriate management approaches and benefits from further characterization and<br />understanding of the variability within a single nematode species. Electrophoretic<br />focusing of malic dehydrogenase, α-glycerophosphate dehydrogenase and<br />esterase for seven populations of Meloidogyne spp. by polyacrylamide gels was<br />tested. These populations included 1, 2 and 4 populations of M. javanica and M.<br />arenaria race I and II, respectively. Malic Dehydrogenase profiles obtained by<br />polyacrylamide-gel electrophoresis indicated that differences between Meloidogyne<br />arenaria and M. javanica were more pronounced than were differences between<br />races or populations of M. arenaria. The enzyme patterns of the two species varied<br />even though the nematodes were propagated on the same host plant (Rutgers<br />tomato).<br /><br />https://ejaj.journals.ekb.eg/article_57479_966c1362dc8a974fa25d4a5d45cefae6.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Long-term effect of Tylenchulus semipenetrans on citrus tree quality in reclaimed land of Egypt53665748210.21608/ejaj.2016.57482ENM.Abd-ElgawadPhytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, EgyptF.KouraPhytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, EgyptS.MontasserDepartment of Agricultural Zoology and Nematology, Faculty of Agriculture, Al-Azhar University, Egypt,M.HammamPhytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, EgyptAhmedEl-bahrawyInstitute for Sustainable Plant Protection, National Council of Research, Bari, Italy.Journal Article20191107Citrus fruit yield is considered a short-term response to the citrus nematode,<br />Tylenchulus semipenetrans damage and therefore this study addressed parameters<br />measuring overall appearance of the sampled trees. Soil and roots from 20 trees in<br />each of three mature: 'banzaheer' lemon, 'Baladi' mandarin, and ‘Navel’ orange and<br />one immature: ‘Navel’ orange orchards, all grafted on sour orange rootstock, in<br />Egypt were sampled for the citrus nematode, T. semipenetrans, in February 2011 to<br />investigate their correlations with tree parameters (tree height and vigor, canopy<br />diameter, and trunk circumference) that reflect long-term nematode damage of the<br />sampled trees. The correlation between pairs of these four parameters were always<br />positive and generally with high significant levels. Each of the four parameters was<br />not significantly correlated with fruit yield of the mature trees but each of tree height<br />and vigor, and canopy diameter was correlated with fruit yield of the immature Navel<br />orange. The relationships between pairs of these biotic parameters and T.<br />semipenetrans population density in fibrous roots and soil were not consistent.<br />Eleven edaphic factors in mandarin and mature orange orchards were measured<br />and compared. Both soils were dominated by rough grainy particles of sand. Levels<br />of potassium, calcium, phosphorus, ammonium and nitrate as well as soil solution<br />pH, organic matter content and electric conductivity in the soil samples were<br />significantly (P ≤ 0.01) different between the mandarin and orange orchards. To<br />achieve better economic revenue of citriculture, local tackling of T. semipenetrans<br />problem in newly reclaimed areas of Egypt was discussed.<br /><br />https://ejaj.journals.ekb.eg/article_57482_b84bdf3d826ae2b733837b90f833c766.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Nematicidal properties of some algal aqueous extracts against root-knot nematode, Meloidogyne incognita in vitro67785748410.21608/ejaj.2016.57484ENAhmedNour El-DeenNematology Research Unit, Agricultural Zoology Dept., Faculty of Agriculture, Mansoura University, EgyptBiology Dept., Faculty of Science, Taif University, Saudi ArabiaAhmedIssaDepartment of Botany, Faculty of Science, Assiut University, Assiut , EgyptBiology Dept., Faculty of Science, Taif University, Saudi ArabiaJournal Article20191107The effectiveness of aqueous extracts derived from nine algal species at<br />different concentrations on egg hatching and mortality of Meloidogyne incognita<br />(Kofoid and White) Chitwood juveniles after various exposure times were<br />determined in vitro. Results indicated that Enteromorpha flexuosa at the<br />concentration of 80% was the best treatment for suppressing the egg hatching with<br />value of 2 % after 5 days of exposure, followed by Dilsea carnosa extract (3%) and<br />Codium fragile (4%) at the same concentration and exposure time. Likewise,<br />application of C. fragile, D. carnosa , E. flexuosa and Cystoseira myrica extracts at<br />the concentrations of 80 and 60% were highly toxic to the nematodes, killing more<br />than 90 % of nematode larva after 72 hours of exposure while the others gave quite<br />low mortalities. The characteristic appearances in shape of the nematodes killed by<br />C. fragile, D. carnosa , C. myrica, E. flexuosa and Sargassum muticum was<br />sigmoid (Σ-shape) with some curved shape; whereas, the nematodes killed by other<br />algal species mostly followed straight or bent shapes. The present study proved that<br />four species of algae C. fragile, D. carnosa, C. myrica and E. flexuosa could be<br />used for the bio-control of root-knot nematodes.<br /><br />https://ejaj.journals.ekb.eg/article_57484_028d59078e007eb70d59acaeb82d652a.pdfEgyptian Society for AgronematologyEgyptian Journal of Agronematology1110-615815120160101Efficacy of some Bioagents, Algal Species and Plant Extracts in Controlling Meloidogyne Incognita on Yield of Grape Vines in Egypt79945748510.21608/ejaj.2016.57485ENSamaaShawkyNematode Research Department, Plant Pathology Research Institute, Agricultural Research Center,
Giza, EgyptAhlamAl-GhonaimyPlant Protection Department, Nematology Unit, Desert Research Center, Cairo, EgyptEmanHammadNematode Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, EgyptJournal Article20191107These experiments were conducted to control the root-knot nematodes,<br />Meloidogyne incognita under both greenhouse and field conditions in superior<br />seedless vineyards and its reflection on yield. The field experiment was carried out<br />in a private vineyard (El Wady El Faregh) in Alexandria-Cairo road (kilo 78). Seven<br />treatments were used as follows: two bioagents (Bacillus subtilis and Trichoderma<br />harzianum), two blue green strains algal species (Cyanobacteria) (Spirulina<br />platensis and Oscillatoria sp.) and two plant aqueous extracts (Ambrosia maritime<br />and Eucalyptus globules) at three concentrations, and in comparison with oxamyl<br />(24% EC) were used to control Meloidogyne incognita on superior seedless<br />vineyards on grapes under both greenhouse and field conditions. Bioagents, algal<br />species and plant extracts were added to the soil under vines one time weekly for<br />three weeks after bud burst under both greenhouse and field conditions.<br />The most effective treatments in controlling root-knot nematodes;<br />Meloidogyne incognita were both Spirulina platensis and Oscillatoria sp whereas<br />the least effective was suspension of Eucalyptus globules under both greenhouse<br />and field conditions. Both Spirulina platensis and Oscillatoria sp acheived the<br />highest reduction effect on number of 2nd stage juveniles in soil, numbers of galls,<br />developmental stages, females, egg masses, egg numbers/egg mass in roots and<br />final population Meloidogyne incognita on grape plants, whereas the suspension of<br />Eucalyptus globules was the least effective treatment under both greenhouse and<br />field conditions. In addition all treatments increased fresh weight of the whole plant<br />over control under greenhouse conditions.<br />Results showed that all the treatments had a significant effect in reducing the<br />total population and build up of root – knot nematodes, Meloidogyne incognita in<br />both soil and roots especially after three months from the time of application. In<br />addition, all treatments had increased the yield of superior seedless grapevines<br />under field conditions.<br /><br />https://ejaj.journals.ekb.eg/article_57485_7223526125e8c0ec638c12da390684fd.pdf