Effect of Water Extracts of some Aromatic and Medicinal Plants on Mortality and Egg Hatching of Root- knot Nematode, Meloidogyne Incognita in Vitro studies

Document Type : Original Article


1 Department of Plant Protection, Sugar Crops, Research Institute, Agriculture Research Center, Giza, Egypt.

2 Department of Agriculture Zoology and Nematology, Faculty of Agriculture, Cairo University, Giza, Egypt.


Water extracts of the ten aromatic and medicinal plant namely, Camphor
(Eucalyptus globulus), Camphor Lemon (E. citriodora), Demssisa (Ambrosia
maritime), Lemon grass (Cymbopgon citratus), Marjoram (Origamum majorana),
Neem (Azadrachta indica), Rosemary (Rosmarimus officinallis) and Thyme
(Thymus vulgaris) as leaves, Chamomile (Matricaria chamomilla) and Daisy
(Calendula officinalis) as leaves and flowers with different concentrations (5, 10 &
15%) of different plants were assessed in vitro against egg hatching and juveniles
(J2s) mortality of root- knot nematodes Meloidogyne incognita in different durations.
Results indicated that eggs inhibition and J2s mortality increased with an increase
in the concentration of all the extracts. Similarly, with an increase in exposure time,
J2s mortality was also increased. Water extracts of all plants showed nematicidal
effect against M. incognita at varying degree. Based on these finding, these plant
extracts at the highest concentration (15%) were divided into 4 main groups i.e.
highly toxic ( 85% mortality or inhibition), consisting of Demssisa, Camphor Lemon
and Thyme, toxic (70 - < 85%) consisting of Daisy and Marjoram, moderately and
lowly toxic groups, consisting of the test plant extracts tested. This finding is
important from the point of view of controlling root- knot nematodes without the use
of chemical nematicides in view of environmental pollution likely to cause. The use
of these plant extracts may be one of the efficient alternatives and cheep methods
of nematode control, that are sale to farmers and inurnment. These may be
replacement to the synthetic dangerous and expensive chemical nematicides in