Efficacy of some Bioagents, Algal Species and Plant Extracts in Controlling Meloidogyne Incognita on Yield of Grape Vines in Egypt

Shawky, Samaa; Al-Ghonaimy, Ahlam; Hammad, Eman

doi:10.21608/ejaj.2016.57485

Efficacy of some Bioagents, Algal Species and Plant Extracts in Controlling Meloidogyne Incognita on Yield of Grape Vines in Egypt

Document Type : Original Article

Authors

¹ Nematode Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

² Plant Protection Department, Nematology Unit, Desert Research Center, Cairo, Egypt

10.21608/ejaj.2016.57485

Abstract

These experiments were conducted to control the root-knot nematodes,
Meloidogyne incognita under both greenhouse and field conditions in superior
seedless vineyards and its reflection on yield. The field experiment was carried out
in a private vineyard (El Wady El Faregh) in Alexandria-Cairo road (kilo 78). Seven
treatments were used as follows: two bioagents (Bacillus subtilis and Trichoderma
harzianum), two blue green strains algal species (Cyanobacteria) (Spirulina
platensis and Oscillatoria sp.) and two plant aqueous extracts (Ambrosia maritime
and Eucalyptus globules) at three concentrations, and in comparison with oxamyl
(24% EC) were used to control Meloidogyne incognita on superior seedless
vineyards on grapes under both greenhouse and field conditions. Bioagents, algal
species and plant extracts were added to the soil under vines one time weekly for
three weeks after bud burst under both greenhouse and field conditions.
The most effective treatments in controlling root-knot nematodes;
Meloidogyne incognita were both Spirulina platensis and Oscillatoria sp whereas
the least effective was suspension of Eucalyptus globules under both greenhouse
and field conditions. Both Spirulina platensis and Oscillatoria sp acheived the
highest reduction effect on number of 2nd stage juveniles in soil, numbers of galls,
developmental stages, females, egg masses, egg numbers/egg mass in roots and
final population Meloidogyne incognita on grape plants, whereas the suspension of
Eucalyptus globules was the least effective treatment under both greenhouse and
field conditions. In addition all treatments increased fresh weight of the whole plant
over control under greenhouse conditions.
Results showed that all the treatments had a significant effect in reducing the
total population and build up of root – knot nematodes, Meloidogyne incognita in
both soil and roots especially after three months from the time of application. In
addition, all treatments had increased the yield of superior seedless grapevines
under field conditions.

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