Effect of Tylenchulus semipenetrans inoculum levels on itsdevelopment, reproduction potential, and damage to infected-Volkamariana seedlings

Abd-Elgawad, Mahfouz; Koura, Faika; Abd El-Wahab, Ahmed; Abd-El-Khair, Hassan; Montasser, Sayed; Hammam, Moustafa

doi:10.21608/ejaj.2014.63687

Effect of Tylenchulus semipenetrans inoculum levels on itsdevelopment, reproduction potential, and damage to infected-Volkamariana seedlings

Document Type : Original Article

Authors

¹ Phytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, Egypt

² Department of Agricultural Zoology and Nematology, Factulty of Agriculture, Al-Azhar University, Egypt.

³ Department of Agricultural Zoology and Nematology, Factulty of Agriculture, Al-Azhar University, Egypt

⁴ Phytopathology Department, National Research Center, El-Tahrir St., Dokki 12622, Giza, Egypt.

10.21608/ejaj.2014.63687

Abstract

Six inocula 0, 5000, 10000, 15000, 20000 and 25000 second stage juveniles
(J2) and males/pot of T. semipenetrans were tested on 1.5-year-old nematode-free
seedlings of Citrus volkameriana. The inoculated seedlings were grown in plastic
pots containing about 5 Kg of sterilized sandy clay soil (2:1) under greenhouse
conditions for a year. Nematode reproduction and host growth were both affected
by the initial inoculum levels. The maximum reduction percentage of shoot length
(38.3%), fresh (25.9%), and dry (44.7%) weight as well as root length (36%) and
fresh weight (30.5%) were recorded at inoculum levels 20000, 20000, 15000, 25000
and 15000 J2 and males/seedling; respectively. On the other hand, the maximum
nematode final population of J2 and males (95515), females (1442), and eggmasses
(820) were obtained at initial inoculum levels of 20000, 20000, and 15000 J2 and
males/seedling; respectively. A negative relationship occurred between the
nematode inoculum level and the rate of reproduction.

Keywords